Difference between revisions of "Part:BBa K1997005"

 
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<partinfo>BBa_K1997005 short</partinfo>
 
<partinfo>BBa_K1997005 short</partinfo>
  
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This is the C-terminal of split Luciferase reporter. It can be used for protein-protein interaction researches.  
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This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "FRB-RBS-FKBP" subpart can be easily replaced using Golden Gate technique with BsaI
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:09, 16 October 2016


sHRP-N->FRB->RBS->FKBP-> sHRP-C

This part is an integrated tool for protein-protein interaction research using split-HRP system as reporter. the "FRB-RBS-FKBP" subpart can be easily replaced using Golden Gate technique with BsaI

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 154
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 396
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1324
    Illegal BsaI.rc site found at 679
    Illegal SapI.rc site found at 1438