Difference between revisions of "Part:BBa K1974012"
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      This year we create a revolutionary system that integrates biological pesticides, an automatic detector, a sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. U2-segestritoxin-Sf1a is coded for the venom of a spider, Segestria florentina. It is under the control of the strong T7 promoter. A 6xHistag is added for further protein purification.
 
      This year we create a revolutionary system that integrates biological pesticides, an automatic detector, a sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. U2-segestritoxin-Sf1a is coded for the venom of a spider, Segestria florentina. It is under the control of the strong T7 promoter. A 6xHistag is added for further protein purification.
  
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<p style="padding-top:20px;"><b>Mechanism of Hv1a</b></p>
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<p style="padding-top:20px;"><b>Mechanism of Sf1a</b></p>
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;According to the reference, Omega-hexatoxin-Hv1a has a structure called ICK(inhibitor cysteine knot).<sup>[1]</sup> This kind of structure contains three disulfide bonds and beta-sheet. With this structure, Hv1a can resist the high temperature, acid base solution and the digest juice of insect gut. Hv1a can bind on insect voltage-gated Calcium channels (CaV1) in the central nervous system, making it paralyze and die eventually.  
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&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;U2-segestritoxin-Sf1a has a structure called ICK(inhibitor cysteine knot). This kind of structure contains four disulfide bonds. With this structure, Sf1a can resist the high temperature, acid-base solution and the digest juice of insect gut. Sf1a can bind on insect voltage-gated sodium channel Site-1, making it paralyze and die eventually.  
  
 
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<p style="padding-top:20px;"><b>Features of Hv1a</b></p>
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<p style="padding-top:20px;"><b>Features of Sf1a</b></p>
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<p style="padding:1px;"><b>1. Non-toxic</b>: Omega-hexatoxin-Hv1a is non-toxic to mammals and Hymenoptera (bees). Since the structure of the target ion channel is different, omega-hexatoxin-Hv1a does not harm mammals and bees. So it is safe to use it as a biological pesticide.</p>
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<p style="padding:1px;"><b>1. Non-toxic</b>: U2-segestritoxin-Sf1a is non-toxic to mammals and bees. Since the structure of the target ion channel is different, U2-segestritoxin-Sf1a does not harm mammals and bees. So it is safe to use it as a biological pesticide.</p>
 
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<p style="padding:1px;"><b>2. Biodegradable</b>: Omega-hexatoxin-Hv1a is a polypeptide so it must degrade over time. After degradation, the toxin will become nutrition in the soil.</p>
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<p style="padding:1px;"><b>2. Biodegradable</b>: The toxin is a peptide, so it must degrade over time. After degradation, the toxin will become nutrition in the soil.</p>
 
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<p style="padding:1px;"><b>3. Species-specific</b>: According to reference, Omega-hexatoxin-Hv1a has specificity to Lepidopteran (moths), Dipteran (flies) and Orthopteran (grasshoppers).</p>
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<p style="padding:1px;"><b>3. Species-specific</b>:According to reference, U2-segestritoxin-Sf1a has specificity to Lepidopteran (moths) and Dipteran (flies). So another insect such as bees will not be killed.</p>
 
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<p style="padding:1px;"><b>4. Eco-friendly</b>: Compare with chemical pesticides, Omega-hexatoxin-Hv1a will not remain in soil and water so that it will not pollute the environment and won’t harm the ecosystem. </p>
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<p style="padding:1px;"><b>4. Eco-friendly</b>: Compare with a chemical pesticide, U2-segestritoxin-Sf1a will not remain in soil and water so that it will not pollute the environment and won’t harm the ecosystem. </p>
  
  

Revision as of 16:09, 16 October 2016

T7Promoter+RBS+Sf1a+linker+6X His-Tag

Introduction:

Figure 1.T7 promoter+RBS+Sf1a+linker+His-tag+terminator

      By ligating the IPTG induced promoter T7 (BBa_ I712074), strong ribosome binding site (BBa_B0034), sf1a, linker, and the 6xHistag (BBa_ K1223006), we can express Sf1a, the toxin by IPTG induction .
      This year we create a revolutionary system that integrates biological pesticides, an automatic detector, a sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. U2-segestritoxin-Sf1a is coded for the venom of a spider, Segestria florentina. It is under the control of the strong T7 promoter. A 6xHistag is added for further protein purification.

Mechanism of Sf1a

      U2-segestritoxin-Sf1a has a structure called ICK(inhibitor cysteine knot). This kind of structure contains four disulfide bonds. With this structure, Sf1a can resist the high temperature, acid-base solution and the digest juice of insect gut. Sf1a can bind on insect voltage-gated sodium channel Site-1, making it paralyze and die eventually.

Features of Sf1a

1. Non-toxic: U2-segestritoxin-Sf1a is non-toxic to mammals and bees. Since the structure of the target ion channel is different, U2-segestritoxin-Sf1a does not harm mammals and bees. So it is safe to use it as a biological pesticide.


2. Biodegradable: The toxin is a peptide, so it must degrade over time. After degradation, the toxin will become nutrition in the soil.


3. Species-specific:According to reference, U2-segestritoxin-Sf1a has specificity to Lepidopteran (moths) and Dipteran (flies). So another insect such as bees will not be killed.


4. Eco-friendly: Compare with a chemical pesticide, U2-segestritoxin-Sf1a will not remain in soil and water so that it will not pollute the environment and won’t harm the ecosystem.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]