Difference between revisions of "Part:BBa K2116011"
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<partinfo>BBa_K2116011 short</partinfo> | <partinfo>BBa_K2116011 short</partinfo> | ||
| − | Promoter norV is the native promoter controlling the nitric oxide reduction operon (norRVW) in ''E. Coli''. | + | Promoter norV is the native promoter controlling the nitric oxide reduction operon (norRVW) in ''E. Coli''. [1] |
We created an AND gate by placing one esabox as roadblock, and one esabox right before the first norR binding site of the PnorV promoter. This second esabox was added to create competitive binding between norR and esaR. | We created an AND gate by placing one esabox as roadblock, and one esabox right before the first norR binding site of the PnorV promoter. This second esabox was added to create competitive binding between norR and esaR. | ||
Revision as of 15:20, 16 October 2016
AND gate regulated by Nitric Oxide and 3OC6HSL
Promoter norV is the native promoter controlling the nitric oxide reduction operon (norRVW) in E. Coli. [1]
We created an AND gate by placing one esabox as roadblock, and one esabox right before the first norR binding site of the PnorV promoter. This second esabox was added to create competitive binding between norR and esaR.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]