Difference between revisions of "Part:BBa K2116011"

Revision as of 15:20, 16 October 2016

AND gate regulated by Nitric Oxide and 3OC6HSL

Promoter norV is the native promoter controlling the nitric oxide reduction operon (norRVW) in E. Coli.

We created an AND gate by placing one esabox as roadblock, and one esabox right before the first norR binding site of the PnorV promoter. This second esabox was added to create competitive binding between norR and esaR.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2116011 short</partinfo>
-rAND gate created by placing one esabox as roadblock, and one esabox right before the first norR binding site of the PnorV promoter. This second esabox is added to create competitive binding between norR and esaR, and improve AND gate behaviour.
+Promoter norV is the native promoter controlling the nitric oxide reduction operon (norRVW) in ''E. Coli''.
+We created an AND gate by placing one esabox as roadblock, and one esabox right before the first norR binding site of the PnorV promoter. This second esabox was added to create competitive binding between norR and esaR.
 <!-- Add more about the biology of this part here