Difference between revisions of "Part:BBa K1974011"
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<p style="padding-top:20px;"><b>Mechanism of Hv1a</b></p> | <p style="padding-top:20px;"><b>Mechanism of Hv1a</b></p> | ||
According to the reference, Omega-hexatoxin-Hv1a has a structure called ICK(inhibitor cysteine knot).<sup>[1]</sup> This kind of structure contains three disulfide bonds and beta-sheet. With this structure, Hv1a can resist the high temperature, acid base solution and the digest juice of insect gut. Hv1a can bind on insect voltage-gated Calcium channels (CaV1) in the central nervous system, making it paralyze and die eventually. | According to the reference, Omega-hexatoxin-Hv1a has a structure called ICK(inhibitor cysteine knot).<sup>[1]</sup> This kind of structure contains three disulfide bonds and beta-sheet. With this structure, Hv1a can resist the high temperature, acid base solution and the digest juice of insect gut. Hv1a can bind on insect voltage-gated Calcium channels (CaV1) in the central nervous system, making it paralyze and die eventually. | ||
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Revision as of 14:32, 16 October 2016
T7 Promoter+RBS+Hv1a+linker+6X His-Tag
Introduction:
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By ligating the IPTG induced promoter T7 (BBa_ I712074), strong ribosome binding site (BBa_B0034), hv1a, linker, and the 6xHistag (BBa_ K1223006), we can express Hv1a, the toxin by IPTG induction.
This year we create a revolutionary system that integrates biological pesticides, an automatic detector, a sprinkler, and IoT. We made a database that contains most of the spider toxins and selected the target toxins by programming. Omega-hexatoxin-Hv1a is coded for the venom of a spider, Hadronyche versuta. It is under the control of the strong T7 promoter. A 6xHistag is added for further protein purification.
Mechanism of Hv1a
According to the reference, Omega-hexatoxin-Hv1a has a structure called ICK(inhibitor cysteine knot).[1] This kind of structure contains three disulfide bonds and beta-sheet. With this structure, Hv1a can resist the high temperature, acid base solution and the digest juice of insect gut. Hv1a can bind on insect voltage-gated Calcium channels (CaV1) in the central nervous system, making it paralyze and die eventually.