Difference between revisions of "Part:BBa K1985006"

 
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NOTOC__
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<partinfo>BBa_K1985006 short</partinfo>
 
<partinfo>BBa_K1985006 short</partinfo>
  
 
MamO is involved in crystal nucleation.  
 
MamO is involved in crystal nucleation.  
  
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Biology:
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[[File:PSB1C3-MamO snap pic.jpeg||400px|thumb|centre|The mamO construct in pSB1C3]]
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MamO is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.  
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<!-- Add more about the biology of this part here
 
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===Usage and Biology===
 
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1985006 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1985000 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  
 
===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1985006 parameters</partinfo>
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<partinfo>BBa_K1985000 parameters</partinfo>
 
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===Usage and Biology===
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Usage: The mamO gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to start the process that should form  magnetite (Fe(II)Fe(III)2O4) crystals when the cell is exposed to iron in solution. It was used to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression. 
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Biology:
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MamO has been described as a crystal nucleation protein. It is part of the magnetosome of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.

Revision as of 14:07, 16 October 2016

NOTOC__ mamO

MamO is involved in crystal nucleation.

The mamO construct in pSB1C3


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 493
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

Usage: The mamO gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to start the process that should form magnetite (Fe(II)Fe(III)2O4) crystals when the cell is exposed to iron in solution. It was used to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.

Biology: MamO has been described as a crystal nucleation protein. It is part of the magnetosome of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.