Difference between revisions of "Part:BBa K2052018"
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<partinfo>BBa_K2052018 short</partinfo>
 
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ButCoaT is an enzyme (Butyryl-CoA:acetate CoA-transferase) (EC 2.8.3.8) (Uniprot:C7GB37) which converts Acetyl-CoA to Butyrate and can be found in Roseburia intestinalis L1-82. The equation of the reaction (acyl-CoA:acetate CoA-transferase) (KEGG Reaction No. : R01179)  is given down below :
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Butanoyl-CoA + Acetate <=> Butanoic acid + Acetyl-CoA
  
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[[File:ButCoaTStructure.gif|none|frame|500px]]
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Figure 1.
  
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Enter a long description of the part so that users of your part know what it is, what it does, and how to use it in their projects.
 
  
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===Usage and Biology===
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===Modeling===
  
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ButCoaT is an enzyme (Butyryl-CoA:acetate CoA-transferase) (EC 2.8.3.8) (Uniprot: C7GB37) that can be found in Roseburia intestinalis L1-82. ButCoaT’s main function iS to convert Acetyl-CoA to Butyrate. The pathway of the reaction (acyl-CoA:acetate CoA-transferase) ( KEGG Reaction No. : R01179 )  is given down below :
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[[File:ButCoaTModeling.jpeg|none|700px|caption]]
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Butanoyl-CoA + Acetate <=> Butanoic acid + Acetyl-CoA
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The figure above shows the increase in ButCoAT molecules over a time span of 10 seconds.  
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[[File:ButyrateModeling.jpeg|none|700px|caption]]
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The figure above shows the molar increase in Butyrate concentration over a time span of 10 seconds.
  
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===Design Notes===
  
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[[File:METU_HS_DENEME2.gif|thumb|Figure1. click on it]]
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E.coli is synthesized suitable for Codon Bias.
  
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In the expression of pet28a (expression vector) , the stop codon is removed for the addition of  HisTag.
  
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===Butyrate===
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===Characterization===
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===Source===
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Roseburia intestinalis L1-82
  
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Butyrate is a four carbon, short chain fatty acid that inhibits cancer. It induces
 
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apoptosis and differentiation, inhibits proliferation of tumorous cells in colon flora. It is produced by the bacterial fermantation of carbohydrates in colon. Butyrate is formed by many pathways that begin with glutarate, Acetyl-CoA and Lysine as it is seen in the picture below. Acetyl-CoA is readily present in the cells so we want to find an enzyme that directly converts it to our treatment, Butyrate which is ButCoAT
 
  
  
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<partinfo>BBa_K2052018 parameters</partinfo>
 
<partinfo>BBa_K2052018 parameters</partinfo>
 
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===References===
 
===References===
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* Figure 1. retrieved from https://swissmodel.expasy.org/templates/4eu8.1
 

Revision as of 13:37, 16 October 2016


Butyryl-CoA:acetate CoA-transferase

ButCoaT is an enzyme (Butyryl-CoA:acetate CoA-transferase) (EC 2.8.3.8) (Uniprot:C7GB37) which converts Acetyl-CoA to Butyrate and can be found in Roseburia intestinalis L1-82. The equation of the reaction (acyl-CoA:acetate CoA-transferase) (KEGG Reaction No. : R01179) is given down below : Butanoyl-CoA + Acetate <=> Butanoic acid + Acetyl-CoA

ButCoaTStructure.gif

Figure 1.


Modeling

caption

The figure above shows the increase in ButCoAT molecules over a time span of 10 seconds.

caption

The figure above shows the molar increase in Butyrate concentration over a time span of 10 seconds.

Design Notes

E.coli is synthesized suitable for Codon Bias.

In the expression of pet28a (expression vector) , the stop codon is removed for the addition of HisTag.

Characterization

Source

Roseburia intestinalis L1-82


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References