Difference between revisions of "Part:BBa K2012016"
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<partinfo>BBa_K2012016 short</partinfo> | <partinfo>BBa_K2012016 short</partinfo> | ||
| − | This generator | + | This generator contains promotor J23117 ,RBS from BBa_J61100,functional chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domain.It can generate weak concentration of CheZ which can be reported by the fluorescence intensity.It is used to detect the relationship of CheZ under different strength of promotor and its swimming ability. |
Promoters with different strength are also constructed as BBa_K2012013[https://parts.igem.org/Part:BBa_K2012013],BBa_K2012014[https://parts.igem.org/Part:BBa_K2012014] | Promoters with different strength are also constructed as BBa_K2012013[https://parts.igem.org/Part:BBa_K2012013],BBa_K2012014[https://parts.igem.org/Part:BBa_K2012014] | ||
<p>We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately<br/> the image are as following </p> | <p>We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately<br/> the image are as following </p> | ||
Revision as of 13:14, 16 October 2016
A weak CheZ generator with fusion GFP reporter
This generator contains promotor J23117 ,RBS from BBa_J61100,functional chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domain.It can generate weak concentration of CheZ which can be reported by the fluorescence intensity.It is used to detect the relationship of CheZ under different strength of promotor and its swimming ability. Promoters with different strength are also constructed as BBa_K2012013[1],BBa_K2012014[2]
We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately
the image are as following
Figure 1.CL1 control was observed under light field and fluorescence field
.
Figure 2.E.coli with BBa_K2012016 was observed under light field and fluorescence field;
Figure 3. The swarming plate of E.coli K12 cheZ lacing strain strain with different strength expression of cheZ-GFP
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 715
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1362