Difference between revisions of "Part:BBa K1231000:Experience"

 
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===User Reviews===
 
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<partinfo>BBa_K1231000 AddReview 3</partinfo>
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<I>iGEM Dundee 2016</I>
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The function of this part was characterised in the hope of designing a pH-responsive system. We hooked up this <i>asr</i> promomoter to an excellent reporter system (<partinfo>BBa_E0840</partinfo>) and characterised its activity under this new composite biobrick (<partinfo>BBa_K1962014</partinfo>). The system showed some pH-dependent activity.
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We also hooked up the <i>asr</i> promoter to biobricks encoding immunity proteins towards colicins. This should give some degree of regulation of transcription of the Im-Ia immunity protein (standard biobrick <partinfo> BBa_K1962001</partinfo> and composite part <partinfo>BBa_K1962015</partinfo>) and the Im-E3 immunity protein (standard biobrick <partinfo>BBa_K1962004</partinfo> and composite part <partinfo>BBa_K1962016</partinfo>).
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Revision as of 11:40, 16 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1231000

User Reviews

UNIQ2c464a9066a9cf83-partinfo-00000000-QINU

•••

iGEM Dundee 2016

The function of this part was characterised in the hope of designing a pH-responsive system. We hooked up this asr promomoter to an excellent reporter system (BBa_E0840) and characterised its activity under this new composite biobrick (BBa_K1962014). The system showed some pH-dependent activity.

We also hooked up the asr promoter to biobricks encoding immunity proteins towards colicins. This should give some degree of regulation of transcription of the Im-Ia immunity protein (standard biobrick No part name specified with partinfo tag. and composite part BBa_K1962015) and the Im-E3 immunity protein (standard biobrick BBa_K1962004 and composite part BBa_K1962016).


UNIQ2c464a9066a9cf83-partinfo-00000008-QINU