Difference between revisions of "Part:BBa K2020042:Design"
 
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===Design Notes===
 
===Design Notes===
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tRNA has 77 amino acids. The ncAA will be attached to the 3' end. On the contrary to aminoacylation with natural amino acids, the acceptor minihelix alone is a substrate for aminoacylation with ncAA's.
 
  
 
===Source===
 
===Source===
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===References===
 
===References===
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*Zhang et al, 2002, Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine
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#Zhang et al, 2002, Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine
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*Steer et al, 1999, Major Anticodon-binding Region Missing from an Archaebacterial tRNA Synthetase
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#Kobayashi et al, 2003, Structural basis for the orthogonal tRNA specificities of tyrosyl-tRNA synthetasees for genetic code expansion
 +
#Schultz et al, 2006, A Genetically Encoded Photocaged Tyrosine

Latest revision as of 11:06, 16 October 2016


Mj-tRNA with amber anticodon for incorporating ncAA in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

wild type tyrosyl Methanococcus janaschii tRNA:Synthetase-pair with anticodon changed to Amber Anticodon CTA. Obtained via iGEM Team 2014 Austin, Texas.

References

  1. Zhang et al, 2002, Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine
  2. Kobayashi et al, 2003, Structural basis for the orthogonal tRNA specificities of tyrosyl-tRNA synthetasees for genetic code expansion
  3. Schultz et al, 2006, A Genetically Encoded Photocaged Tyrosine