Difference between revisions of "Part:BBa K2020042:Design"
 
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===Design Notes===
 
===Design Notes===
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Since tRNA and Synthetases have own promotors and since they are frequently used in low copy plasmids, you should assemble tRNA and Syntehtases on a plasmid (e.g. pACYC) which fits your needs.
 
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Team Aachen 2016 ( http://2016.igem.org/Team:Aachen ) used tRNA in pACYC with p15A-ORI, Ipp-Promoter, Terminator, Gent-Resistance. Together with an ncAA-Synthetase (For example: BBa_K2020050) with own promoter and Terminator.
 
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===Source===
 
===Source===
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===References===
 
===References===
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#Zhang et al, 2002, Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine
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#Kobayashi et al, 2003, Structural basis for the orthogonal tRNA specificities of tyrosyl-tRNA synthetasees for genetic code expansion
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#Schultz et al, 2006, A Genetically Encoded Photocaged Tyrosine

Latest revision as of 11:06, 16 October 2016


Mj-tRNA with amber anticodon for incorporating ncAA in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

wild type tyrosyl Methanococcus janaschii tRNA:Synthetase-pair with anticodon changed to Amber Anticodon CTA. Obtained via iGEM Team 2014 Austin, Texas.

References

  1. Zhang et al, 2002, Structure-based design of mutant Methanococcus jannaschii tyrosyl-tRNA synthetase for incorporation of O-methyl-L-tyrosine
  2. Kobayashi et al, 2003, Structural basis for the orthogonal tRNA specificities of tyrosyl-tRNA synthetasees for genetic code expansion
  3. Schultz et al, 2006, A Genetically Encoded Photocaged Tyrosine