Difference between revisions of "Part:BBa K1932006"

Revision as of 08:04, 16 October 2016

This device is constructed to express TAT-apoptin fused with sec2.

This device was cloned to express the fusion protein, TAT-apoptin and the sec2 peptide coding sequence was included to increase the secretion of the protein. Among the subparts, BBa_K1932000 is a strong promoter to regulate the expression of exogenous protein in Bifidobacterium. BBa_K1932001 is included to enhance the stability of the device in Bifidobacterium. BBa_K1932002 is the sequence of the signal peptide, sec2, that can direct the export of the protein from Bididobacterium. BBa_K1932004 encodes the TAT-apoptin fusion protein, which can induce the apoptosis specifically in the cancer cells.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1059

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 <partinfo>BBa_K1932006 short</partinfo>
-This device was cloned to express the fusion protein, TAT-apoptin and the sec2 peptide coding sequence was included to increase the secretion of the protein. Among the subparts, BBa_1932000 is a strong promoter to regulate the expression of exogenous protein in Bifidobacterium. BBa_1932001 is included to enhance the stability of the device in Bifidobacterium. BBa_193202 is the sequence of the signal peptide, sec2, that can direct the export of the protein from Bididobacterium. BBa_1932004 encodes the TAT-apoptin fusion protein, which can induce the apoptosis specifically in the cancer cells.
+This device was cloned to express the fusion protein, TAT-apoptin and the sec2 peptide coding sequence was included to increase the secretion of the protein. Among the subparts, BBa_K1932000 is a strong promoter to regulate the expression of exogenous protein in Bifidobacterium. BBa_K1932001 is included to enhance the stability of the device in Bifidobacterium. BBa_K1932002 is the sequence of the signal peptide, sec2, that can direct the export of the protein from Bididobacterium. BBa_K1932004 encodes the TAT-apoptin fusion protein, which can induce the apoptosis specifically in the cancer cells.
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