Difference between revisions of "Part:BBa K1898100:Design"
Line 9: Line 9:
 
We removed one point mutation from the cDNA with mutagenesis done by MissionBiotech.  
 
We removed one point mutation from the cDNA with mutagenesis done by MissionBiotech.  
 
Primers were designed to remove the stop codon and were synthesized by Tri-I biotech.
 
Primers were designed to remove the stop codon and were synthesized by Tri-I biotech.
−
 
+
forward: 5' ATATgAATTCgCggCCgCTTCTAgATggACgTgACCATCCAgCA 3' (44)
−
 
+
reverse: 5' ATATCTgCAgCggCCgCTACTAgTATTAggACgAgggAgCCgAg 3' (44)
  
 
===Source===
 
===Source===

Revision as of 06:28, 16 October 2016


ch25h, cholesterol 25 hydroxylase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We removed one point mutation from the cDNA with mutagenesis done by MissionBiotech. Primers were designed to remove the stop codon and were synthesized by Tri-I biotech. forward: 5' ATATgAATTCgCggCCgCTTCTAgATggACgTgACCATCCAgCA 3' (44) reverse: 5' ATATCTgCAgCggCCgCTACTAgTATTAggACgAgggAgCCgAg 3' (44)

Source

cDNA of ch25h was ordered from Sino BIological Inc

References