Difference between revisions of "Part:BBa K1871010"
 
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<partinfo>BBa_K1871000 short</partinfo>
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<partinfo>BBa_K1871010 short</partinfo>
  
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[[File:alphaamylase.jpg|200 px|right|Alpha amylase protein]]
 
  
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This is an AMY gene obtained from a Bacillus strain of bacteria. It produces an enzyme that hydrolyzes starch and degrades it into monosaccarides.
  
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This is an alpha amylase gene obtained from ''Bacillus amyloliquefaciens''. It is flanked by a Kozak sequence and ADH1 terminator.
 
  
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====Kozak Sequence====
 
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This part initiates translation from eukaryotic mRNA and is cloned between a promoter and coding region to facilitate translation.
 
  
 
====Native Alpha Amylase Secretion Sequence====
 
====Native Alpha Amylase Secretion Sequence====
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A signal sequence which directs the protein to be secreted.
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This is the ''Bacillus amyloliquefaciens'' alpha amylase signal sequence, which directs the protein to be secreted.
  
 
====Alpha Amylase====
 
====Alpha Amylase====
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Obtained from Bacillus amyloliquefaciens, α-Amylase is a protein enzyme that hydrolyses alpha bonds of large, alpha-linked polysaccharides, such as starch and glycogen.
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α-Amylase is a protein enzyme that hydrolyses alpha bonds of long alpha-linked polysaccharides, such as glycogen.
  
 
====ADH1 Terminator====
 
====ADH1 Terminator====
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This part is the terminator region from yeast alcohol dehydrogenase (ADH1) gene. This stops the RNA polymerase from transcribing the RNA sequence.
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This part is the terminator region from yeast alcohol dehydrogenase gene.  
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<!-- Add more about the biology of this part here
 
 
====Usage and Biology====
 
====Usage and Biology====
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This part was proved functional by the 2015 BRHS iGEM Team. Please click the below link to see their results.
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http://2015.igem.org/Team:BroadRun-NorthernVA/Results
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1871000 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K1871010 SequenceAndFeatures</partinfo>
  
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K1871000 parameters</partinfo>
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<partinfo>BBa_K1871010 parameters</partinfo>
 
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Latest revision as of 03:15, 16 October 2016

Coding Sequence AMY1


This is an AMY gene obtained from a Bacillus strain of bacteria. It produces an enzyme that hydrolyzes starch and degrades it into monosaccarides.


Native Alpha Amylase Secretion Sequence

This is the Bacillus amyloliquefaciens alpha amylase signal sequence, which directs the protein to be secreted.

Alpha Amylase

α-Amylase is a protein enzyme that hydrolyses alpha bonds of long alpha-linked polysaccharides, such as glycogen.

ADH1 Terminator

This part is the terminator region from yeast alcohol dehydrogenase gene.


Usage and Biology

This part was proved functional by the 2015 BRHS iGEM Team. Please click the below link to see their results. http://2015.igem.org/Team:BroadRun-NorthernVA/Results


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 8
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1436
    Illegal NgoMIV site found at 1451
  • 1000
    COMPATIBLE WITH RFC[1000]