Difference between revisions of "Part:BBa K1972006"

Revision as of 01:45, 16 October 2016

dszC (a monooxygenase gene ), E.coli-optimized

DszC is a monoxygenase which can not only convert dibenzothiophene (DBT) to DBT-sulfoxide (DBTO) but also convert DBT-sulfoxide (DBTO) to DBTsulfone (DBTO2)during the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP). And DszC require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working.

This single flavinreductase gene was synthesized whith E.coli codon optimized by Generay and the sequence was found in NCBI (GenBank Accession number L37363.1)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 168
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 397
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K1972006 short</partinfo>
-DszA, DszB, DszC and dszD are required by the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP). And three successive oxidation steps require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working.
+DszC is a monoxygenase which can not only convert dibenzothiophene (DBT) to DBT-sulfoxide (DBTO) but also convert DBT-sulfoxide (DBTO) to DBTsulfone (DBTO2)during the 4S pathway, in which dibenzothiophene (DBT) undergoes three successive oxidation steps and one a hydrolytic step leading to the formation of 2-hydroxybiphenyl (2HBP). And DszC require reducing equivalents (FMNH2) supplied by a flavinreductase (DszD) while working.
 This single flavinreductase gene was synthesized whith E.coli codon optimized by Generay and the sequence was found in NCBI (GenBank Accession number L37363.1)