Difference between revisions of "Part:BBa K2042014"

Latest revision as of 22:30, 15 October 2016

RBS + PhaC (sp. MBEL 6-19) + RBS + PCT (CP)

This is the sequence of the PLA producing operon. This sequence is optimized for P.putida and is constituted of two gene propionate CoA transferase (Pct) and PHA synthase (PhaC). Pct contain a mutation that allows him to take lactate as the acceptor and convert it efficiently into lactyl-CoA. The PhaC gene contains 4 amino acid mutation which were found to polymerize monomer of lactyl-coA into PLA homopolymer. This gene is placed upstream in the sequence and close of the promoter because polymerization is the bottleneck of the PLA producing system.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2386
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1073
Illegal NgoMIV site found at 1352
Illegal AgeI site found at 148
Illegal AgeI site found at 461
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 2792
Illegal BsaI site found at 3263

@@ Line 4: / Line 4: @@
 This is the sequence of the PLA producing operon. This sequence is optimized for P.putida and is constituted of two gene propionate CoA transferase (Pct) and PHA synthase (PhaC). Pct contain a mutation that allows him to take lactate as the acceptor and convert it efficiently into lactyl-CoA. The PhaC gene contains 4 amino acid mutation which were found to polymerize monomer of lactyl-coA into PLA homopolymer.
-This gene is placed upstream in the sequence closer of the promoter because polymerization is the bottleneck of the PLA producing system.
+This gene is placed upstream in the sequence and close of the promoter because polymerization is the bottleneck of the PLA producing system.
 <!-- Add more about the biology of this part here