Difference between revisions of "Part:BBa K2042013"
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− | This is the sequence of the operon constituted of | + | This is the sequence of the operon constituted of PHA synthase 1 gene from Pseudomonas resinovorans and Propionate CoA transferase from Clostridum propionicum. The operon use lactate as substract, convert it into lactyl-coA and do it polymerisation into PLA. |
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Latest revision as of 22:19, 15 October 2016
RBS +PhaC_resinovorans + RBS + Pct
This is the sequence of the operon constituted of PHA synthase 1 gene from Pseudomonas resinovorans and Propionate CoA transferase from Clostridum propionicum. The operon use lactate as substract, convert it into lactyl-coA and do it polymerisation into PLA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 100
Illegal BamHI site found at 2386
Illegal XhoI site found at 185
Illegal XhoI site found at 1043 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1073
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 809
Illegal BsaI site found at 2792
Illegal BsaI site found at 3263