Difference between revisions of "Part:BBa K2042013"

 
 
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This is the sequence of the operon constituted of PHA synthase 1 gene from Pseudomonas resinovorans and Propionate CoA transferase from Clostridum propionicum. The operon use lactate as substract, convert it into lactyl-coA and do it polymerisation into PLA.
  
 
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Latest revision as of 22:19, 15 October 2016


RBS +PhaC_resinovorans + RBS + Pct

This is the sequence of the operon constituted of PHA synthase 1 gene from Pseudomonas resinovorans and Propionate CoA transferase from Clostridum propionicum. The operon use lactate as substract, convert it into lactyl-coA and do it polymerisation into PLA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 100
    Illegal BamHI site found at 2386
    Illegal XhoI site found at 185
    Illegal XhoI site found at 1043
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 809
    Illegal BsaI site found at 2792
    Illegal BsaI site found at 3263