Difference between revisions of "Part:BBa K1951009:Design"

(→‎Design Notes)
(→‎Design Notes)
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High level flagellin expression:
 
High level flagellin expression:
 
This biobrick is composed of two parts:
 
This biobrick is composed of two parts:
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* [https://parts.igem.org/Part:BBa_K880005 BBa_K880005] A strong promoter, strong RBS combination specifically conceived and tested for high expression levels of proteins in <i>E.coli</i> (this part is itself composed of sub-parts [https://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] and [https://parts.igem.org/Part:BBa_B0034 BBa_B0034]
+
* [https://parts.igem.org/Part:BBa_K1951006 BBa_K880006] A strong promoter, strong RBS combination specifically conceived and tested for high expression levels of proteins in <i>E.coli</i> (this part is itself composed of sub-parts [https://parts.igem.org/Part:BBa_J23100 BBa_J23100 ] and [https://parts.igem.org/Part:BBa_B0034 BBa_B0034]
−
* [https://parts.igem.org/Part:BBa_K1951005 BBa_K1951005] the FliC coding sequence designed by our team for high level expression in <i>E.coli</i> with sequence optimization.
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* [https://parts.igem.org/Part:BBa_K1951005 BBa_K1951005] the FliC coding sequence designed by our team for high level expression in <i>Desulfovibrio vulgaris</i> with sequence optimization.
  
 +
===Sequence optimization===
 
We found the raw sequence from online data base '''QUEL DATA BASE YOANN????''
 
We found the raw sequence from online data base '''QUEL DATA BASE YOANN????''
 +
 +
===Promoter and RBS selection===
 +
 +
As the purpose of this part is high level protein expression we selected the part [https://parts.igem.org/Part:BBa_K1951006 BBa_K880006] to drive expression of our coding sequence. We chose this part as:
 +
* it combines a strong promotor and a RBS (so avoiding the separate incorporation of 2 parts).
  
 
===Source===
 
===Source===

Revision as of 22:12, 15 October 2016


FliC Desulfovibrio producer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 362
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 461


Design Notes

High level flagellin expression: This biobrick is composed of two parts:

  • BBa_K880006 A strong promoter, strong RBS combination specifically conceived and tested for high expression levels of proteins in E.coli (this part is itself composed of sub-parts BBa_J23100 and BBa_B0034
  • BBa_K1951005 the FliC coding sequence designed by our team for high level expression in Desulfovibrio vulgaris with sequence optimization.

Sequence optimization

We found the raw sequence from online data base 'QUEL DATA BASE YOANN????

Promoter and RBS selection

As the purpose of this part is high level protein expression we selected the part BBa_K880006 to drive expression of our coding sequence. We chose this part as:

  • it combines a strong promotor and a RBS (so avoiding the separate incorporation of 2 parts).

Source

We used previous biobrick :

- BBa_K880005

- BBa_K1951006

References