Difference between revisions of "Part:BBa K2042012"

Latest revision as of 21:49, 15 October 2016

RBS + PhaC_sp. MBEL 6-19

This is the sequence of Pseudomonas putida ribosom binding site followed by Pseudomonas sp. MBEL 6-19 PHA synthase 1 gene. The gene is codon optimized for P.putida and contain four mutation (E130D/S325T/S477G/Q481K) allowing to take the (D)-lactyl-CoA and produce the PLA homopolymer. It is used in the biobrick BBa_K2042014

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1073
Illegal NgoMIV site found at 1352
Illegal AgeI site found at 148
Illegal AgeI site found at 461
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2042012 short</partinfo>
-This is the sequence of Pseudomonas putida ribosom binding site followed by Pseudomonas sp. MBEL 6-19 PHA synthase 1 gene. The gene is codon optimized for P.putida and contain four mutation (E130D/S325T/S477G/Q481K) allowing to take the (D)-lactyl-CoA and produce the PLA homopolymer. It is used in the biobrick BBa_K2042014
+This is the sequence of Pseudomonas putida ribosom binding site followed by Pseudomonas sp. MBEL 6-19 PHA synthase 1 gene. The gene is codon optimized for P.putida and contain four mutation (E130D/S325T/S477G/Q481K) allowing to take the (D)-lactyl-CoA and produce the PLA homopolymer. It is used in the biobrick
 <html>
 <a href="https://parts.igem.org/Part:BBa_K2042014" title="BBa_K2042014">BBa_K2042014</a><br>