Difference between revisions of "Part:BBa K2042012"
 
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<partinfo>BBa_K2042012 short</partinfo>
 
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This is the sequence of Pseudomonas putida ribosom binding site followed by Pseudomonas sp. MBEL 6-19 PHA synthase 1 gene. The gene is codon optimized for P.putida and contain four mutation (E130D/S325T/S477G/Q481K) allowing to take the (D)-lactyl-CoA and produce the PLA homopolymer.
 
  
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This is the sequence of Pseudomonas putida ribosom binding site followed by Pseudomonas sp. MBEL 6-19 PHA synthase 1 gene. The gene is codon optimized for P.putida and contain four mutation (E130D/S325T/S477G/Q481K) allowing to take the (D)-lactyl-CoA and produce the PLA homopolymer. It is used in the biobrick
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<a href="https://parts.igem.org/Part:BBa_K2042014" title="BBa_K2042014">BBa_K2042014</a><br>
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Latest revision as of 21:49, 15 October 2016


RBS + PhaC_sp. MBEL 6-19

This is the sequence of Pseudomonas putida ribosom binding site followed by Pseudomonas sp. MBEL 6-19 PHA synthase 1 gene. The gene is codon optimized for P.putida and contain four mutation (E130D/S325T/S477G/Q481K) allowing to take the (D)-lactyl-CoA and produce the PLA homopolymer. It is used in the biobrick BBa_K2042014

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1073
    Illegal NgoMIV site found at 1352
    Illegal AgeI site found at 148
    Illegal AgeI site found at 461
  • 1000
    COMPATIBLE WITH RFC[1000]