Difference between revisions of "Part:BBa K2065007"

 
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<partinfo>BBa_K2065007 short</partinfo>
 
<partinfo>BBa_K2065007 short</partinfo>
  
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Under consturction
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The SmallBiT-CT52 BioBrick is part of a split luciferase system. This luciferase system consists of two parts, namely SmallBiT and LargeBiT. It is a ATP-independent luciferase, that produces high intensity bioluminescence. The total weight of this luciferase system is 19.1 kDa. The NanoLuc system consists of 171 amino acids. When they dimerize, this means when they come into close proximity of each other, one can see bioluminescence. The emission peak of this NanoBiT system is around 460 nanometers. This is blue light.
  
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===Usage and Biology===
 
===Usage and Biology===
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<p>
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SmallBiT is linked to CT52 with a GGS10-linker, so it actually consists of three subparts. SmallBiT is 33 basepairs long and thus consists of 11 amino acids. The GGS10-linker that is used, consists of 30 amino acids, it is a ten-times repeated sequence of glycine-glycine-serine amino acids. This complex is linked to CT52. 
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This protein consist of the last 52 amino acids of the C-terminal region of the H+-ATPase. The N-terminus of CT52 is a free end, which can be used to link proteins to. The binding between CT52 and T14-3-3 is stabilized by the small molecule Fusicoccin. When Fusicoccin is present, the affinity of CT52 for T14-3-3 is increased in 30 fold with respect to the situation when Fusicoccin is not present [3]. The binding interaction with Fusicoccin is reversibel, this means CT52 will dissociate from the scaffold.
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</p>
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<p>
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This means that the CT52 -SmallBiT complex will move towards the scaffold protein, after a small molecule, Fusicoccin, is added.
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Three different variants of the CT52-protein were created with the help of the Rosetta software package. Each mutation in the CT52 protein has its complementary mutation in the T14-3-3 protein. Because these mutations were found with the help of the Rosetta software package, the quality of these mutations have to be verified.
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One variant of the C52 protein was already known and tested. This mutation functioned as a positive control in the verification process of the other mutations.
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</p>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2065007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2065007 SequenceAndFeatures</partinfo>

Revision as of 21:28, 15 October 2016


CT52-LargeBiT

The SmallBiT-CT52 BioBrick is part of a split luciferase system. This luciferase system consists of two parts, namely SmallBiT and LargeBiT. It is a ATP-independent luciferase, that produces high intensity bioluminescence. The total weight of this luciferase system is 19.1 kDa. The NanoLuc system consists of 171 amino acids. When they dimerize, this means when they come into close proximity of each other, one can see bioluminescence. The emission peak of this NanoBiT system is around 460 nanometers. This is blue light.

Usage and Biology

SmallBiT is linked to CT52 with a GGS10-linker, so it actually consists of three subparts. SmallBiT is 33 basepairs long and thus consists of 11 amino acids. The GGS10-linker that is used, consists of 30 amino acids, it is a ten-times repeated sequence of glycine-glycine-serine amino acids. This complex is linked to CT52. This protein consist of the last 52 amino acids of the C-terminal region of the H+-ATPase. The N-terminus of CT52 is a free end, which can be used to link proteins to. The binding between CT52 and T14-3-3 is stabilized by the small molecule Fusicoccin. When Fusicoccin is present, the affinity of CT52 for T14-3-3 is increased in 30 fold with respect to the situation when Fusicoccin is not present [3]. The binding interaction with Fusicoccin is reversibel, this means CT52 will dissociate from the scaffold.

This means that the CT52 -SmallBiT complex will move towards the scaffold protein, after a small molecule, Fusicoccin, is added. Three different variants of the CT52-protein were created with the help of the Rosetta software package. Each mutation in the CT52 protein has its complementary mutation in the T14-3-3 protein. Because these mutations were found with the help of the Rosetta software package, the quality of these mutations have to be verified. One variant of the C52 protein was already known and tested. This mutation functioned as a positive control in the verification process of the other mutations.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 730
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]