Difference between revisions of "Part:BBa K1936000"
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<partinfo>BBa_K1936000 short</partinfo> | <partinfo>BBa_K1936000 short</partinfo> | ||
− | ALERT !!!Under construction !! | + | ALERT !!!Under construction !! |
− | PDZ domains are commonly found in signaling proteins and play important roles in recruitment and scaffold association. The engineered PDZ (ePDZ) domain is designed to interact with LOV2 peptide (blue light inducible system). We utilized the binding of ePDZ to the J-alpha-helix of LOV2 to recruit ePDZ-mCherry-BaxS184E fusion protein to the outer mitochondrial membrane (BBa_K1936001). [1] | + | <!-- Add more about the biology of this part here |
+ | ===Usage and Biology=== | ||
+ | |||
+ | PDZ domains are commonly found in signaling proteins and play important roles in recruitment and scaffold association. | ||
+ | The engineered PDZ (ePDZ) domain is designed to interact with LOV2 peptide (blue light inducible system). We utilized the binding of ePDZ to the J-alpha-helix of LOV2 to recruit ePDZ-mCherry-BaxS184E fusion protein to the outer mitochondrial membrane (OOM) (<a href=" https://parts.igem.org/Part:BBa_K1936001/"> BBa_K1936001</a>). [1] | ||
The human Bax protein acts as cofactor for the tumor suppressor gene p53 that is associated with acceleration of apoptosis. | The human Bax protein acts as cofactor for the tumor suppressor gene p53 that is associated with acceleration of apoptosis. | ||
It translocates from the cytosol to the outer mitochondrial membrane (OMM) where it participates in the formation of mitochondrial apoptosis-induced channels (MAC). Through those channels cytochrome c can be released from the inner membrane space to the cytosol which leads to apoptosome formation and therefore triggers cell death. | It translocates from the cytosol to the outer mitochondrial membrane (OMM) where it participates in the formation of mitochondrial apoptosis-induced channels (MAC). Through those channels cytochrome c can be released from the inner membrane space to the cytosol which leads to apoptosome formation and therefore triggers cell death. | ||
− | The BaxS184E mutant has been shown to not migrate from cytosol to OMM on its own | + | The BaxS184E mutant has been shown to not migrate from cytosol to OMM on its own. To induce apoptosis by expressing BaxS184E recruitment to the OMM is necessary. [2] |
The red fluorescent protein mCherry is used to track the fusion protein localization inside a cell by microscopy. | The red fluorescent protein mCherry is used to track the fusion protein localization inside a cell by microscopy. | ||
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Revision as of 17:24, 15 October 2016
ePDZ-mCherry-BAX184E
ALERT !!!Under construction !!
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1327
Illegal BamHI site found at 1796
Illegal XhoI site found at 1331 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]