Difference between revisions of "Part:BBa K1992002"
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Tar is a chemoreceptor found in the bacterium E-coli which mediates chemotaxis toward Aspartic acid and away from Nickel and Cobalt (1). Using designs from bioinformatic tool, a noval chemoreceptor, which mediates chemotaxis toward Histamine, was formed by induce directed point mutations to the Tar ligand-binding domain (LBD) (link to K777000 part). This noval receptor is a part of the S.Tar platform.
 
Tar is a chemoreceptor found in the bacterium E-coli which mediates chemotaxis toward Aspartic acid and away from Nickel and Cobalt (1). Using designs from bioinformatic tool, a noval chemoreceptor, which mediates chemotaxis toward Histamine, was formed by induce directed point mutations to the Tar ligand-binding domain (LBD) (link to K777000 part). This noval receptor is a part of the S.Tar platform.
  
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===Usage and Biology===
 
===Usage and Biology===
 
Histamine is a derivative of Histidine, which is also an amino acid as the native Tar ligand. The motivation to mediates chemotaxis toward Histamine is duo to it’s presence in food poison, especially in rotten fish.   
 
Histamine is a derivative of Histidine, which is also an amino acid as the native Tar ligand. The motivation to mediates chemotaxis toward Histamine is duo to it’s presence in food poison, especially in rotten fish.   
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==Design considerations==
  
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TEEEMPPPPP
 
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<partinfo>BBa_K1992011 parameters</partinfo>
 
  
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===Functional Parameters===
 
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<partinfo>BBa_K1992011 parameters</partinfo>
 
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<NAME>
 
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<p>
 
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Introduction
 
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Methods
 
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Results
 
  
 
[[File:His_9_t0.png|450px|thumb|left|alt text]]
 
[[File:His_9_t0.png|450px|thumb|left|alt text]]

Revision as of 17:03, 15 October 2016

Histamin-Tar receptor

Novel Histamine-Tar chemoreceptor in E.coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1355
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 184

Introduction

Tar is a chemoreceptor found in the bacterium E-coli which mediates chemotaxis toward Aspartic acid and away from Nickel and Cobalt (1). Using designs from bioinformatic tool, a noval chemoreceptor, which mediates chemotaxis toward Histamine, was formed by induce directed point mutations to the Tar ligand-binding domain (LBD) (link to K777000 part). This noval receptor is a part of the S.Tar platform.

Usage and Biology

Histamine is a derivative of Histidine, which is also an amino acid as the native Tar ligand. The motivation to mediates chemotaxis toward Histamine is duo to it’s presence in food poison, especially in rotten fish. The new chemoreceptor enable chemotactic attractant response to Histamine, althogth Histamin chemoreceptor could be found in human cells, this is the first time this receptor conducted in bacterium cell.


Design considerations

alt text

</p>

conclusion 1)