Difference between revisions of "Part:BBa K2012007"
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the whole part contains CheZ fused with GFP,which can show the concentration of CheZ. This coding part do not have its RBS and promoter. Users have to add their preferred RBS and promoter by using biobrick assembly or other suitable assembly. | the whole part contains CheZ fused with GFP,which can show the concentration of CheZ. This coding part do not have its RBS and promoter. Users have to add their preferred RBS and promoter by using biobrick assembly or other suitable assembly. | ||
We have proved the function of emitting green light under certain exciting light and the function of improving motility by transform it into a <span style="font-style:italic">cheZ</span> lacking strain, CL1. | We have proved the function of emitting green light under certain exciting light and the function of improving motility by transform it into a <span style="font-style:italic">cheZ</span> lacking strain, CL1. | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 16:54, 15 October 2016
CheZ fussed with a CG linker and reporter GFP(BBa_E0040)
the whole part contains CheZ fused with GFP,which can show the concentration of CheZ. This coding part do not have its RBS and promoter. Users have to add their preferred RBS and promoter by using biobrick assembly or other suitable assembly.
We have proved the function of emitting green light under certain exciting light and the function of improving motility by transform it into a cheZ lacking strain, CL1.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1301