Difference between revisions of "Part:BBa K2012009"
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<p>Figure 1.Light field of <i>E.coli</i> was grown on semi-solid medium with BBa_K2012009.Figure 2.Fluorescence field of <i>E.coli</i> was grown on semi-solid medium with BBa_K2012009;</p>
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<p>Figure 1.<i>E.coli</i> with BBa_K2012009 was observed under light field.Figure 2.<i>E.coli</i> with BBa_K2012009 was observed under fluorescence_field;</p>
 
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Revision as of 16:01, 15 October 2016


RBS(J61100) and CheZ with fused with a GS linker and GFP in C teminal

 These parts are present in plasmid pSB1C3, but there is also a constitutive promoter (J23100-derived) inserted into the XbaI site. So, for example, the EcoRI/PstI region of part K2012009 reads:
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Biobrick 5'            XbaI                                            J23100                                    XbaI             K2012009                      Biobrick 3'
gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctaga...................................actagtagcggccgctgcag

 

This feature in no way prevents the use of these parts in standard Biobrick assembly. Normal prefix insertion into EcoRI/XbaI will delete this promoter element. Suffix insertion into SpeI/PstI will retain this promoter, but it can of course be removed later by a prefix insertion.

Note also that the base 5' to the SpeI site is allowed to float in these parts and is therefore rarely "T". The "G" downstream of the XbaI site obeys the standard. Because the database does not permit variation at this position, the predicted sequences of composite parts derived from these parts will be incorrect at this position.(some content are cited from J61100 part(BBa_J61100))

Figure 1.E.coli with BBa_K2012009 was observed under light field.Figure 2.E.coli with BBa_K2012009 was observed under fluorescence_field;


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1321