Difference between revisions of "Part:BBa K1830002:Experience"

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===Applications of BBa_K1830002===
 
===Applications of BBa_K1830002===
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BBa K1830002 is stated as the signal peptide of Usp45, however,after thorough literature search we found that SPusp45 is only 27 amino acids long, but not the current version of BBa_K1830002. We confirmed that the 27 amino acids long SPusp45 signal peptide can be used for secreting proteins out of the ''L.lactis'' cell. It should be fused to the N-terminal of the protein of interest.
 
  
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iGEMers who want to use the SPusp45, they should chose the BBa K1654007 part, we have verified that BBa K1654007 is functional in protein secretion.
 
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===Group===
 
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TMMU-China-2016
 
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===Author===
 
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Yifei Lu
 
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===Summary===
 
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We characterized that the 27 amino acids long SPusp45 signal peptide but not BBa K1654007 is the right choice that can be used to secret proteins out of L. lactis.
 
 
===User Reviews===
 
===User Reviews===
 
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<partinfo>BBa_K1830002 AddReview number</partinfo>
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<partinfo>BBa_K1830002 AddReview 1</partinfo>
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<I>Username</I>
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<I>TMMU-China-2016 </I>
 
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Enter the review inofrmation here.
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BBa K1830002 is stated as the signal peptide of Usp45, however,after thorough literature search we found that SPusp45 is only 27 amino acids long, but not the current version of BBa_K1830002. We confirmed that the 27 amino acids long SPusp45 signal peptide can be used for secreting proteins out of the L.lactis cell. It should be fused to the N-terminal or C-terminal of the protein of interest.
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iGEMers who want to use the SPusp45, they should choose the BBa K1654007 part, we have verified that BBa K1654007 is functional in protein secretion.
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Latest revision as of 14:52, 15 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1830002

User Reviews

UNIQ6d46bedceeb29126-partinfo-00000000-QINU

•

TMMU-China-2016

BBa K1830002 is stated as the signal peptide of Usp45, however,after thorough literature search we found that SPusp45 is only 27 amino acids long, but not the current version of BBa_K1830002. We confirmed that the 27 amino acids long SPusp45 signal peptide can be used for secreting proteins out of the L.lactis cell. It should be fused to the N-terminal or C-terminal of the protein of interest.

iGEMers who want to use the SPusp45, they should choose the BBa K1654007 part, we have verified that BBa K1654007 is functional in protein secretion.

UNIQ6d46bedceeb29126-partinfo-00000002-QINU