Difference between revisions of "Part:BBa K2066016"
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<partinfo>BBa_K2066016 short</partinfo> | <partinfo>BBa_K2066016 short</partinfo> | ||
| − | to be | + | In order to characterize the transfer function of a promoter with and without RiboJ, it is necessary to induce the activity of that promoter. plLacO-1 and pTac are both repressed by the lac repressor, which in turn is repressed by IPTG— hence IPTG will induce the activity of plLacO-1 and pTac. This part is designed for gBlock synthesis by IDT. It is then meant to be cloned into the plasmid form of WM16_014 or WM16_015 to create WM16_018 and WM16_019, which replicate the plasmids in Lou et al. 2012, “Ribozyme-based insulator parts buffer synthetic circuits from genetic context”. |
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Revision as of 14:31, 15 October 2016
Constitutive Lac Repressor on UNS Standard
In order to characterize the transfer function of a promoter with and without RiboJ, it is necessary to induce the activity of that promoter. plLacO-1 and pTac are both repressed by the lac repressor, which in turn is repressed by IPTG— hence IPTG will induce the activity of plLacO-1 and pTac. This part is designed for gBlock synthesis by IDT. It is then meant to be cloned into the plasmid form of WM16_014 or WM16_015 to create WM16_018 and WM16_019, which replicate the plasmids in Lou et al. 2012, “Ribozyme-based insulator parts buffer synthetic circuits from genetic context”.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 47
Illegal NheI site found at 70 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]