Difference between revisions of "Part:BBa K2012009"
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<partinfo>BBa_K2012009 short</partinfo> | <partinfo>BBa_K2012009 short</partinfo> | ||
− | Bacteria movement related gene CheZ which fused with a GS linker and GFP in C teminal. We will use this part to charactise the expression of CheZ. | + | Bacteria movement related gene CheZ which fused with a GS linker and GFP in C teminal. We will use this part to charactise the expression of CheZ. These parts are present in plasmid pSB1C3, but there is also a constitutive promoter(J23100-derived) |
+ | So, for example, the EcoRI/PstI region of part K2012009 reads: | ||
+ | [Biobrick5' XbaI J23100 XbaI '''K2012009''' Biobrick 3' | ||
+ | gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctaga'''sequence part...'''actagtagcggccgctgcag] | ||
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Revision as of 14:28, 15 October 2016
RBS(J61100) and CheZ with fused with a GS linker and GFP in C teminal
Bacteria movement related gene CheZ which fused with a GS linker and GFP in C teminal. We will use this part to charactise the expression of CheZ. These parts are present in plasmid pSB1C3, but there is also a constitutive promoter(J23100-derived) So, for example, the EcoRI/PstI region of part K2012009 reads: [Biobrick5' XbaI J23100 XbaI K2012009 Biobrick 3' gaattcgcggccgcttctagaGTTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGCTtctagasequence part...actagtagcggccgctgcag]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1321