Difference between revisions of "Part:BBa K1934061:Experience"
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===Applications of BBa_K1934061=== | ===Applications of BBa_K1934061=== | ||
| − | + | This part has been transferred into e. coli NM522 for expression. After 6 hours at 37° IPTG has been added to the final concentration of 1 mM. The expression culture was grown overnight at 30°. We the used instructions provided on the NiNTA columns kit from Quigen to purify the protein. | |
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K1934061 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1934061 StartReviews</partinfo> | ||
Revision as of 13:51, 15 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1934061
This part has been transferred into e. coli NM522 for expression. After 6 hours at 37° IPTG has been added to the final concentration of 1 mM. The expression culture was grown overnight at 30°. We the used instructions provided on the NiNTA columns kit from Quigen to purify the protein.
User Reviews
UNIQ33a7345555fe9bd1-partinfo-00000000-QINU UNIQ33a7345555fe9bd1-partinfo-00000001-QINU