Difference between revisions of "Part:BBa K1897012:Design"

Latest revision as of 12:54, 15 October 2016

Listeriolysin O CDS

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1380
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The sequence is codon optimised for expression in Escherichia coli.

Source

Listeriolysin O (LLO) is derived from Listeria monocytogenes and is initially obtained from BBa_K177026. However, after sequencing, the results obtained for sequencing of BBa_K177026 did not appear to match that given on the page. We went on to synthesize new LLO (for BBa_K1897013) with the results obtained from sequencing.

References

Kayal, S., & Charbit, A. (2006). Listeriolysin O: a key protein of Listeria monocytogenes with multiple functions. FEMS microbiology reviews, 30(4), 514-529.

Vadia, S., Arnett, E., Haghighat, A. C., Wilson-Kubalek, E. M., Tweten, R. K., & Seveau, S. (2011). The pore-forming toxin listeriolysin O mediates a novel entry pathway of L. monocytogenes into human hepatocytes. PLoS Pathog, 7(11), e1002356.

@@ Line 8: / Line 8: @@
 ===Design Notes===
 The sequence is codon optimised for expression in <i>Escherichia coli</i>.
 ===Source===
@@ Line 16: / Line 14: @@
 ===References===
+Kayal, S., & Charbit, A. (2006). Listeriolysin O: a key protein of Listeria monocytogenes with multiple functions. <i>FEMS microbiology reviews, 30</i>(4), 514-529.
+Vadia, S., Arnett, E., Haghighat, A. C., Wilson-Kubalek, E. M., Tweten, R. K., & Seveau, S. (2011). The pore-forming toxin listeriolysin O mediates a novel entry pathway of L. monocytogenes into human hepatocytes. <i>PLoS Pathog, 7</i>(11), e1002356.