Difference between revisions of "Part:BBa K1893003"

Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1893003 short</partinfo>
 
<partinfo>BBa_K1893003 short</partinfo>
The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. RhlR is activated by C4-HSL. Included in the construct is the RhlR-activated promoter pRhl upstream a promoter sequence which includes GFP
+
The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. RhlR is activated by C4-HSL. Included in the construct is the RhlR-activated promoter pRhl upstream a reporter sequence which includes GFP
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 12:39, 15 October 2016


Rhl receiver with GFP reporter (RhlR+pRhl+GFP) The gene encoding the transcriptional activator RhlR is downstream a constitutive Anderson promoter, j23101. RhlR is activated by C4-HSL. Included in the construct is the RhlR-activated promoter pRhl upstream a reporter sequence which includes GFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776
    Illegal BsaI.rc site found at 1658