Difference between revisions of "Part:BBa K1897012:Design"
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===Design Notes=== | ===Design Notes=== | ||
The sequence is codon optimised for expression in <i>Escherichia coli</i>. | The sequence is codon optimised for expression in <i>Escherichia coli</i>. | ||
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===Source=== | ===Source=== | ||
Revision as of 11:57, 15 October 2016
Listeriolysin O CDS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1380
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence is codon optimised for expression in Escherichia coli.
Source
Listeriolysin O (LLO) is derived from Listeria monocytogenes and is initially obtained from BBa_K177026. However, after sequencing, the results obtained for sequencing of BBa_K177026 did not appear to match that given on the page. We went on to synthesize new LLO (for BBa_K1897013) with the results obtained from sequencing.