Difference between revisions of "Part:BBa K2110104"
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We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. | We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows. | ||
https://static.igem.org/mediawiki/parts/thumb/f/f3/T--Tianjin--partdataR90A.png/800px-T--Tianjin--partdataR90A.png | https://static.igem.org/mediawiki/parts/thumb/f/f3/T--Tianjin--partdataR90A.png/800px-T--Tianjin--partdataR90A.png | ||
| + | |||
| + | We used the relative fluorescence units to represent the expression level of enzymes. | ||
| + | https://static.igem.org/mediawiki/2016/2/28/T--Tianjin--part-R90Aex.png | ||
| + | |||
| + | Finally we got the relative activities of enzymes. | ||
| + | https://static.igem.org/mediawiki/2016/2/29/T--Tianjin--part-R90Abar.png | ||
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Revision as of 10:17, 15 October 2016
PETase site-directed mutant R90A
This is one of the site-directed mutant of the PETase gene, we change the 90th amino acid from R to A. The module is the Saccharomyces cerevisiae codon optimization version of PETase gene. We use overlap PCR to obtain this mutant.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 79
Illegal BglII site found at 289 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Results
We detect the absorption peak of culture medium after culturing with PET film added for 2d. The absorption degree-wavelength curve is as follows.
We used the relative fluorescence units to represent the expression level of enzymes.
Finally we got the relative activities of enzymes.
