Difference between revisions of "Part:BBa K1937001"

 
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<partinfo>BBa_K1937001 short</partinfo>
 
<partinfo>BBa_K1937001 short</partinfo>
  
Part: BBa_K1937001 (pSBBsOK-mini)
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=<b>Part: BBa_K1937001 (pSBBsOK-mini)</b>=
(Chassis E. coli, carrier plasmid pSB1C3, part destined for use in Bacillus subtilis)
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(Chassis <i>E. coli</i>, carrier plasmid pSB1C3, part destined for use in <i>Bacillus subtilis</i>)
 
Length: 5857 bp
 
Length: 5857 bp
  
This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has an ampicillin resistance for cloning in E.coli and kanamycin resistance for selection in B. subtilis.
 
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France)
 
  
We successfully transformed E. coli and selected clones on ampicillin. Likewise, we successfully transformed B. subtilis and selected clones on kanamycine. The plasmid sequence has been entirely verified by sequencing. The capacity to sub-clone in this new backbone has been demonstrated (part BBa_K1937004, BBa_K1937006, BBa_K1937008 and BBa_K1937010).
+
This part is an expression vector for Bacillus subtilis. It is replicative both in <i>E.coli</i> and <i>B.subtilis</i>. It has an ampicillin resistance for cloning in <i>E.coli</i> and kanamycin resistance for selection in <i>B. subtilis</i>.
 +
This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France).
 +
 
 +
 
 +
We successfully transformed <i>E. coli</i> and selected clones on ampicillin. Likewise, we successfully transformed <i>B. subtilis</i> and selected clones on kanamycine. The plasmid sequence has been entirely verified by sequencing. The capacity to sub-clone in this new backbone has been demonstrated (part BBa_K1937004, BBa_K1937006, BBa_K1937008 and BBa_K1937010).
  
  

Revision as of 09:01, 15 October 2016


pSBBsOK-mini, a small replicative plasmid for Bacillus subtilis

Part: BBa_K1937001 (pSBBsOK-mini)

(Chassis E. coli, carrier plasmid pSB1C3, part destined for use in Bacillus subtilis) Length: 5857 bp


This part is an expression vector for Bacillus subtilis. It is replicative both in E.coli and B.subtilis. It has an ampicillin resistance for cloning in E.coli and kanamycin resistance for selection in B. subtilis. This BioBrick is a part developed by the Toulouse 2016 iGEM team (http://2016.igem.org/Team:Toulouse_France).


We successfully transformed E. coli and selected clones on ampicillin. Likewise, we successfully transformed B. subtilis and selected clones on kanamycine. The plasmid sequence has been entirely verified by sequencing. The capacity to sub-clone in this new backbone has been demonstrated (part BBa_K1937004, BBa_K1937006, BBa_K1937008 and BBa_K1937010).


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 257
    Illegal suffix found in sequence at 285
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 257
    Illegal NheI site found at 279
    Illegal SpeI site found at 286
    Illegal PstI site found at 300
    Illegal NotI site found at 263
    Illegal NotI site found at 293
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 257
    Illegal BglII site found at 4814
    Illegal BamHI site found at 330
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 257
    Illegal suffix found in sequence at 286
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 257
    Illegal XbaI site found at 272
    Illegal SpeI site found at 286
    Illegal PstI site found at 300
    Illegal NgoMIV site found at 1877
    Illegal AgeI site found at 4425
    Illegal AgeI site found at 5387
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 55
    Illegal BsaI.rc site found at 1701
    Illegal BsaI.rc site found at 3140
    Illegal BsaI.rc site found at 5656
    Illegal SapI site found at 618
    Illegal SapI.rc site found at 4638