Difference between revisions of "Part:BBa K1884009"

Revision as of 08:58, 15 October 2016

PSAD Promoter+GalAD-CRY2+UAS+FNR(fumarate and nitrate reduction)+PSAD Terminator

The device we constructed this year is one of the functional plasmids in the light-mediated expression system,followed the example of yeast two-hybrid system. With BD-CIB1 which is constructed in the other device(BBa_K1884007), light-mediated expression system, followed the example of yeast two-hybrid system, will be activated and starting to transcript Ferredoxin-NADP(+)Reductase(BBa_K1884006) which is on the 3 prime end of Upstream activating sequence(BBa_K1884004) in green algae

Biology

PSAD Promoter

AD-CRY2

UAS

FNR

PSAD Terminator

Usage

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 3123
Illegal NotI site found at 3221
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1560
Illegal BglII site found at 2019
Illegal BamHI site found at 2498
Illegal XhoI site found at 3052
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1444
Illegal AgeI site found at 2173
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1796
Illegal BsaI.rc site found at 1205
Illegal SapI.rc site found at 1313
Illegal SapI.rc site found at 3384

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K1884009 short</partinfo>
+The device we constructed this year is one of the functional plasmids in the light-mediated expression system,followed the example of yeast two-hybrid system. With BD-CIB1 which is constructed in the other device(BBa_K1884007), light-mediated expression system, followed the example of yeast two-hybrid system, will be activated and starting to transcript Ferredoxin-NADP(+)Reductase(BBa_K1884006) which is on the 3 prime end of Upstream activating sequence(BBa_K1884004) in green algae
+===Biology===
+PSAD Promoter
+AD-CRY2
+UAS
+FNR
+PSAD Terminator
+===Usage===