Difference between revisions of "Part:BBa K1995024"
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IHG (INP-N-GFP-HG)
 
IHG (INP-N-GFP-HG)
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Based on BBa_K1995000 (BHG), we added INP-N and GFP to the upstream of BHG. Using INP-N, we anchored GFP and BHG out of the outer membrane of E.coli in order to increase the binding efficiency and using GFP to detect the concentration of cupric and mercuric ions.
  
 
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<!-- Add more about the biology of this part here

Revision as of 06:46, 15 October 2016


IHG (INP-N-GFP-HG)

IHG (INP-N-GFP-HG) Based on BBa_K1995000 (BHG), we added INP-N and GFP to the upstream of BHG. Using INP-N, we anchored GFP and BHG out of the outer membrane of E.coli in order to increase the binding efficiency and using GFP to detect the concentration of cupric and mercuric ions.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 429
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1181
    Illegal SapI.rc site found at 238