Difference between revisions of "Part:BBa K2070023"

Revision as of 02:25, 15 October 2016

Constitutive ecf16 generator

This part is used to constitutively generate the trigger required for the activation of the Toehold rbs in part BBa_K2070018 to generate GFP. The trigger and toehold together form the Toehold System.

Detailed Explanation of the Toehold System

Ribosomes cannot bind to the Toehold RBS under normal conditions. Therefore, proteins cannot be made because mRNAs cannot be translated even when the gene is transcribed. For a ribosome to bind to a toehold RBS, the existence of a specific short RNA named trigger RNA is necessary.

The previous gene in which the toehold RBS stops transcription becomes activated as shown below when trigger RNA is present, and will be translated. Functioning DNA is represented as yellow one.

Reference

Green, A., Silver, P., Collins, J., & Yin, P. (2014, 11). Toehold Switches: De-Novo-Designed Regulators of Gene Expression. Cell, 159(4), 925-939. doi:10.1016/j.cell.2014.10.002

Sequence and Features