Difference between revisions of "Part:BBa K2100006"
Line 3: | Line 3: | ||
<partinfo>BBa_K2100006 short</partinfo> | <partinfo>BBa_K2100006 short</partinfo> | ||
− | + | eYFP is a fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter. The promoter and gene are encoded within an expression vector backbone with an SV40 terminator site after the eYFP gene and an ampicillin resistant gene. | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 02:17, 15 October 2016
pENTR mKate
eYFP is a fluorescent protein constituitively expressed with the hEF1a (human elongation factor 1 alpha) promoter. The promoter and gene are encoded within an expression vector backbone with an SV40 terminator site after the eYFP gene and an ampicillin resistant gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 647
Illegal SapI.rc site found at 29