Difference between revisions of "Part:BBa K2070001"
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<partinfo>BBa_K2070001 short</partinfo> | <partinfo>BBa_K2070001 short</partinfo> | ||
| − | Ecf16 is an extracytoplasmic function (ECF) sigma factor from <i>Pseudomonas entomophila </i> used to activate the ecf16 promoter (<partinfo>BBa_K2070000</partinfo>). | + | Ecf16 is an extracytoplasmic function (ECF) sigma factor taken from <i>Pseudomonas entomophila </i> used to activate the ecf16 promoter (<partinfo>BBa_K2070000</partinfo>). The sigma factor is found to be highly orthogonal to its corresponding promoter and can be inactivated by antisigma factor16 (<partinfo>BBa_K2070002</partinfo>). |
===Source=== | ===Source=== | ||
Latest revision as of 01:04, 15 October 2016
ecf16
Ecf16 is an extracytoplasmic function (ECF) sigma factor taken from Pseudomonas entomophila used to activate the ecf16 promoter (BBa_K2070000). The sigma factor is found to be highly orthogonal to its corresponding promoter and can be inactivated by antisigma factor16 (BBa_K2070002).
Source
Pseudomonas entomophila L48
References
Rhodius, V. A., Segall-Shapiro, T. H., Sharon, B. D., Ghodasara, A., Orlova, E., Tabakh, H., Voigt, C. A. (2014, 04). Design of orthogonal genetic switches based on a crosstalk map of s, anti- s, and promoters. Molecular Systems Biology, 9(1), 702-702. doi:10.1038/msb.2013.58
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 177
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 372
- 1000COMPATIBLE WITH RFC[1000]