Difference between revisions of "Part:BBa K1897028"

 
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<partinfo>BBa_K1897028 short</partinfo>
 
<partinfo>BBa_K1897028 short</partinfo>
  
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The construct contains one of the synthetic lldR promoters designed by team ETH-Zurich, IGEM 2015 (BBa_K1847008) with a weaker RBS (BBa_B0033), followed by a CDS of sfGFP and a lambda t0 terminator.
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The construct contains one of the synthetic lldR promoters designed by team ETH-Zurich, IGEM 2015 ([[Part: BBa_K1847008]]) with a weaker RBS flanked by non-coding sequences ([[Part: BBa_K1897031]]), followed by a CDS of sfGFP ([[Part: BBa_K1897033]]) and a lambda t0 terminator ([[Part: BBa_K1897030]]).
  
  

Revision as of 21:57, 14 October 2016


p70-33-sfGFP-Terminator

The construct contains one of the synthetic lldR promoters designed by team ETH-Zurich, IGEM 2015 (Part: BBa_K1847008) with a weaker RBS flanked by non-coding sequences (Part: BBa_K1897031), followed by a CDS of sfGFP (Part: BBa_K1897033) and a lambda t0 terminator (Part: BBa_K1897030).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 589
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]