Difference between revisions of "Part:BBa K2088672"
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<partinfo>BBa_K2088672 short</partinfo>
 
<partinfo>BBa_K2088672 short</partinfo>
  
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This composite part uses a promoter induced by copper ions(BBa_K2088001&#65289;&#65292; riboJ&#65288;BBa_K2066506&#65289;&#65292; mhbD with native rbs&#65288;BBa_K2088667&#65289;, mhbH with native rbs(BBa_K2088670), mhbI with native rbs(BBa_K2088669), mhbM with native rbs(BBa_K2088668), and a double terminator(BBa_B0015).
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The composite part contains copA  promoter,  riboJ , a gene cluster mhbDHIM , and a terminator. The promoter is induced by copper ion and transcribe downstream genes. The riboJ between copA and the gene cluster can decrease interference of one part type （promoter）on another（protein coding sequence）. The gene cluster mhbDHIM contains four connected genes which can degrade 3-hydroxybenzoate to the Fumarate and Pyruvate step by step. After experienting, we found that the device can degrade the 3-hydroxybenzoate completely in short time. Also, engineering bacteria with the device grows better in the medium with 3-hydroxybenzoate than Klebsiella pneumoniae M5a1which is capable of utilizing 3-hydroxybenzoate via gentisate, and can tolerate higher concentration of 3- hydroxybenzoate.In conclusion, the device can help the E.coli degrade the 3- hydroxybenzoate effectively. 
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This part is used to degrade 3-hydroxybenzoate(3-HBA) to fumarate and pyruvate via the gentisate pathway induced by  copper ions.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:39, 14 October 2016


copA + riboJ +mhbDHIM + terminator

The composite part contains copA promoter, riboJ , a gene cluster mhbDHIM , and a terminator. The promoter is induced by copper ion and transcribe downstream genes. The riboJ between copA and the gene cluster can decrease interference of one part type （promoter）on another（protein coding sequence）. The gene cluster mhbDHIM contains four connected genes which can degrade 3-hydroxybenzoate to the Fumarate and Pyruvate step by step. After experienting, we found that the device can degrade the 3-hydroxybenzoate completely in short time. Also, engineering bacteria with the device grows better in the medium with 3-hydroxybenzoate than Klebsiella pneumoniae M5a1which is capable of utilizing 3-hydroxybenzoate via gentisate, and can tolerate higher concentration of 3- hydroxybenzoate.In conclusion, the device can help the E.coli degrade the 3- hydroxybenzoate effectively.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2518
    Illegal BamHI site found at 2904
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 480
    Illegal NgoMIV site found at 754
    Illegal NgoMIV site found at 2708
    Illegal NgoMIV site found at 3845
    Illegal AgeI site found at 816
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3038