Difference between revisions of "Part:BBa K1947029"
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+ | __NOTOC__ | ||
+ | <partinfo>BBa_K1947029 short</partinfo> | ||
+ | TEV protease may bring some impure proteins. We use intein to replace the TEV site. The M. xenopi GyrA intein provides a paradigm for a minimal protein splicing element. Intein can be cleaved by DTT. DTT as reducing agent protects the free thiol groups of the protein from oxidation and so it doesn’t bring foreign substance. As a result, intein can achieve a better effect of purification than TEV protease. | ||
+ | |||
+ | |||
+ | <!-- Add more about the biology of this part here | ||
+ | ===Usage and Biology=== | ||
+ | |||
+ | <!-- --> | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
+ | <partinfo>BBa_K1947029 SequenceAndFeatures</partinfo> | ||
+ | |||
+ | |||
+ | <!-- Uncomment this to enable Functional Parameter display | ||
+ | ===Functional Parameters=== | ||
+ | <partinfo>BBa_K1947029 parameters</partinfo> | ||
+ | <!-- --> |
Revision as of 14:16, 14 October 2016
This part is used to improve the protein purification.
TEV protease may bring some impure proteins. We use intein to replace the TEV site. The M. xenopi GyrA intein provides a paradigm for a minimal protein splicing element. Intein can be cleaved by DTT. DTT as reducing agent protects the free thiol groups of the protein from oxidation and so it doesn’t bring foreign substance. As a result, intein can achieve a better effect of purification than TEV protease.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1230
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1150