Difference between revisions of "Part:BBa K1920006"

Revision as of 13:45, 14 October 2016

pBAD-RBS-lysis-B0015

pBAD is an E.coli promoter that is induced by L-arabinose.In the absence of arabinose, the repressor protein AraC (BBa_I13458) binds to the AraI1 operator site of pBAD and the upstream operator site AraO2, blocking transcription [1]. In the presence of arabinose, AraC binds to it and changes its conformation such that it interacts with the AraI1 and AraI2 operator sites, permitting transcription [1].[2] Using pBAD as the promoter of lysis allows us to control lysis process. However, under the enviroment of high concentration of glucose, pBAD will be inhibited[2]. Hence the this is a promoter can be controlled with two usual substance, glucose and arabinose. Yet, in order to make glucose effective to pBADthe concentration need to be higher than 0.01%.

[1]Schleif, R.Regulation of the l-arabinose operon of Escherichia coli. Trends in Genetics(2000) [2] I-Son Ng, et al., Simultaneous release of recombinant cellulases introduced by coexpressing colicin E7 lysis in Escherichia coli, Biotechnology and Bioprocess Engineering (2016)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 However, under the enviroment of high concentration of glucose, pBAD will be inhibited[2]. Hence the this is a promoter can be controlled with two usual substance, glucose and arabinose.
 Yet, in order to make glucose effective to pBADthe concentration need to be higher than 0.01%.
+[1]Schleif, R.Regulation of the l-arabinose operon of Escherichia coli. Trends in Genetics(2000)
+[2] I-Son Ng, et al., Simultaneous release of recombinant cellulases introduced by coexpressing colicin E7 lysis in Escherichia coli, Biotechnology and Bioprocess Engineering (2016)