Difference between revisions of "Part:BBa K1981101:Design"

 
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===Design Notes===
 
===Design Notes===
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No point mutation was performed.
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A cytosine was added at the 3' of the original sequence of <i>plsr</i> promoter since the original 3' sequence of <i>plsr</i> is "AATTC". It is quite possible that an illegal EcoRI restriction site will be assembled if the DNA fragment asserted at 3' of <i>plsr</i> ends with a G. For example, an illegal EcoRI restriction site would be assembled if we use the original sequence when constructing the standard biobrick since the 5' of standard prefix is G.By inserting a C at 3' of the original sequence, this problem can be solved.
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===Source===
 
===Source===

Latest revision as of 13:18, 14 October 2016


lsr promoter of LuxS/AI-2 signaling pathway in E.coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

A cytosine was added at the 3' of the original sequence of plsr promoter since the original 3' sequence of plsr is "AATTC". It is quite possible that an illegal EcoRI restriction site will be assembled if the DNA fragment asserted at 3' of plsr ends with a G. For example, an illegal EcoRI restriction site would be assembled if we use the original sequence when constructing the standard biobrick since the 5' of standard prefix is G.By inserting a C at 3' of the original sequence, this problem can be solved.

Source

plsr source

References