Difference between revisions of "Part:BBa K1985003"

Revision as of 12:57, 14 October 2016

mamP, his-tagged, signal sequence cleaved

This part is an differentiation on part <a href="https://parts.igem.org/Part:BBa_K1985">BBa_K1985000</a>

Sequence and Features

Assembly Compatibility:

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 __NOTOC__
 <partinfo>BBa_K1985003 short</partinfo>
-Usage: The mamX gene produces a protein that can be used to transfer electrons to iron molecules. It can be used in combination with other mam genes to form an electron transport complex (reference) that should form magnetic magnetite crystals when the cell is exposed to into in solution. It was to assemble a construct of MamO, P, T and X in pSB1A3 for in vivo expression.
+This part is an differentiation on part <a href="https://parts.igem.org/Part:BBa_K1985">BBa_K1985000</a>
-Biology:
-MamX is a proposed cytochrome protein (reference) due to the presence of a heme binding motif within its sequence. It forms a complex with other Mam proteins on the membrane of the native species, Magnetospirillum gryphiswaldense. Together they promote magnetite crystal maturation within an organelle called the magnetosome.
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 ===Usage and Biology===
+This part has a his-tag included so was used to purify the expressed mamP protein. MamP is usually targeted to the membrane, however in this part the targeting sequence has been cleaved and it should instead be targeted to the periplasm. For more information on its biology and usage, see part BBa_K1985000.
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