Difference between revisions of "Part:BBa K1918101:Design"

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===Design Notes===
 
===Design Notes===
−
AIM-the expression of EBFP2N(1-154) would be induced by Dox.
+
AIM: the expression of the EBFP2N(1-154) could be induced by Dox;
 +
ELEMENTS: EBFP2N(1-154), Dox-induced promoter TRE
  
 
===Source===
 
===Source===
−
ligation from [pwx226(EcoRI&PspOMI)][pwx161(WL16049&WL16055)]
+
PCR from the existing plasmid in xie lab
  
 
===References===
 
===References===

Latest revision as of 12:16, 14 October 2016


TRE-Kozak-EBFP2N(1-154)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 859
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 379
    Illegal BamHI site found at 362
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 859
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 379
    Illegal XbaI site found at 859
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

AIM: the expression of the EBFP2N(1-154) could be induced by Dox; ELEMENTS: EBFP2N(1-154), Dox-induced promoter TRE

Source

PCR from the existing plasmid in xie lab

References