Difference between revisions of "Part:BBa K2148010"

 
 
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<partinfo>BBa_K2148010 short</partinfo>
 
<partinfo>BBa_K2148010 short</partinfo>
  
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This is a coding DNA sequence (CDS) for a far-red fluorescent protein which is codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick.  
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This is a coding DNA sequence (CDS) for a far-red fluorescent protein, codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick.  
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    <figure>
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        <img src="https://static.igem.org/mediawiki/2016/0/0b/T--Cambridge-JIC--part--mRas--1.png" width="100%" height="100%" style="float:center">
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        <figcaption>
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        <p style="font-size:11px; text-align:center">Comparison of the colors of fluorescent proteins[1].
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        </p>
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        </figcaption>
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    </figure>
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</html>
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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This is a level-0 Phytobrick conforming to the Phytobrick standard. It is coded as a CDS/CTAG Phytobrick and can be used with other level-0 Phytobricks to create a transcriptional unit that expresses a far-red fluorescent protein for Chlamydomonas reinhardtii chloroplast.
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mRaspberry has excitation and emission maxima at 598 and 625 nm, respectively, and so should not overlap with chlorophyll's spectra. It has not been previously tested in the chloroplast of <i> Chlamydomonas reinhardtii </i>, but has the potential to be a novel reporter for it. The part has been codon-optimised, so its expression should be enhanced.
  
 
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<partinfo>BBa_K2148010 parameters</partinfo>
 
<partinfo>BBa_K2148010 parameters</partinfo>
 
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<!-- Uncomment this to enable Functional Parameter display
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===Characterisation===
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===References===
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1. Zamai M. et al. (2008). Biomarkers and luminescent probes in quantitative biology. Biological and Medical Physics, Biomedical Engineering: Biophotonics (Springer), p. 182.

Latest revision as of 11:56, 14 October 2016


mRasberry C.reinhardtii-CO far-red FP

This is a coding DNA sequence (CDS) for a far-red fluorescent protein, codon-optimised for Chlamydomonas reinhardtii chloroplast. It is coded as a CDS/CTAG Phytobrick.

Comparison of the colors of fluorescent proteins[1].


Usage and Biology

This is a level-0 Phytobrick conforming to the Phytobrick standard. It is coded as a CDS/CTAG Phytobrick and can be used with other level-0 Phytobricks to create a transcriptional unit that expresses a far-red fluorescent protein for Chlamydomonas reinhardtii chloroplast.

mRaspberry has excitation and emission maxima at 598 and 625 nm, respectively, and so should not overlap with chlorophyll's spectra. It has not been previously tested in the chloroplast of Chlamydomonas reinhardtii , but has the potential to be a novel reporter for it. The part has been codon-optimised, so its expression should be enhanced.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 231
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 231
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 231
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 231
  • 1000
    COMPATIBLE WITH RFC[1000]



References

1. Zamai M. et al. (2008). Biomarkers and luminescent probes in quantitative biology. Biological and Medical Physics, Biomedical Engineering: Biophotonics (Springer), p. 182.