Difference between revisions of "Part:BBa K1981001"
Songxinhao (Talk | contribs) |
Songxinhao (Talk | contribs) |
||
Line 3: | Line 3: | ||
<partinfo>BBa_K1981001 short</partinfo> | <partinfo>BBa_K1981001 short</partinfo> | ||
− | Autoinducer 2 import ATP-binding protein LsrA. Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Regulation of uptake and processing of the quorum-sensing AI-2. Responsible for energy coupling to the transport system (Probable). This protein is essential for aerobic growth. In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter. | + | Autoinducer 2 import ATP-binding protein LsrA. Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Regulation of uptake and processing of the quorum-sensing AI-2. Responsible for energy coupling to the transport system (Probable). This protein is essential for aerobic growth. In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the <i>lsr</i> promoter. |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Line 26: | Line 26: | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Construct AI-2 “consumer” by overexpressing the <i>lsrA</i> gene <i>Bacillus amyloliquefaciens LL3</i>. | Construct AI-2 “consumer” by overexpressing the <i>lsrA</i> gene <i>Bacillus amyloliquefaciens LL3</i>. | ||
− | |||
− |
Revision as of 09:21, 14 October 2016
lsrA
Autoinducer 2 import ATP-binding protein LsrA. Part of the ABC transporter complex LsrABCD involved in autoinducer 2 (AI-2) import. Regulation of uptake and processing of the quorum-sensing AI-2. Responsible for energy coupling to the transport system (Probable). This protein is essential for aerobic growth. In the absence of AI-2, repressed by LsrR. Induced by AI-2, via release of the LsrR repressor. In the absence of glucose, induced by cAMP-CRP by direct binding to the upstream region of the lsr promoter.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Construct AI-2 “consumer” by overexpressing the lsrA gene Bacillus amyloliquefaciens LL3.