Difference between revisions of "Part:BBa K1887000:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The lacZ gene from Lactobacillus acidophilus was introduced into the His locus of L. Lactis genome by temperature sensitive vector, which makes the knocked-in strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside). | + | The lacZ gene from ''Lactobacillus acidophilus'' was introduced into the His locus of ''L. Lactis'' genome by temperature sensitive vector, which makes the knocked-in strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside). |
===Source=== | ===Source=== | ||
Revision as of 09:04, 14 October 2016
LacZ
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The lacZ gene from Lactobacillus acidophilus was introduced into the His locus of L. Lactis genome by temperature sensitive vector, which makes the knocked-in strains become blue on plate with X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside).
Source
Using the genomic DNA of Lactobacillus acidophilus as the template, the lacZ gene product is obtained by PCR and cloned into the pSB1C3 plasmid.
References
Simoes-Barbosa, A., Abreu, H., Silva Neto, A., Gruss, A., and Langella, P. (2004). A food-grade delivery system for Lactococcus lactis and evaluation of inducible gene expression. Appl Microbiol Biotechnol 65, 61-67.